Transfer-PCR (TPCR): A highway for DNA cloning and protein engineering

High efficiency transformation of Escherichia coli with plasmids

Hiroaki Inoue, Hiroshi Nojima, Hiroto Okayama · Gene · 1990 · 2.3K citations

Biotechnology, Escherichia Coli, Microbiology +3

Ligation-independent cloning of PCR products (LIC-PCR)

Charalampos Aslanidis, Pieter J. de Jong · Nucleic Acids Research · 1990 · 1.2K citations · Full text

De Novo Protein Design: Fully Automated Sequence Selection

Bassil I. Dahiyat, Stephen L. Mayo · Science · 1997 · 1.1K citations

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Mamie Z. Li, Stephen J. Elledge · Nature Methods · 2007 · 965 citations

Engineering, Genetics, Homologous Recombination +9

A Novel, Highly Stable Fold of the Immunoglobulin Binding Domain of Streptococcal Protein G

Angela M. Gronenborn, David Filpula, Nina Z. Essig et al. · Science · 1991 · 818 citations