Matrix protein of VSV New Jersey serotype containing methionine to arginine substitutions at positions 48 and 51 allows near-normal host cell gene expression

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

Thomas R. Fuerst, Edward G. Niles, F. William Studier et al. · Proceedings of the National Academy of Sciences · 1986 · 2.1K citations · Full text

Life Sciences, Microbiology, Molecular Biology +15

The "megaprimer" method of site-directed mutagenesis.

Gobinda Sarkar, S S Sommer · PubMed · 1990 · 1.2K citations

Life Sciences, Genetics, Molecular Biology +12

Generation of Bovine Respiratory Syncytial Virus (BRSV) from cDNA: BRSV NS2 Is Not Essential for Virus Replication in Tissue Culture, and the Human RSV Leader Region Acts as a Functional BRSV Genome Promoter

Ursula J. Buchholz, Stefan Finke, Karl‐Klaus Conzelmann · Journal of Virology · 1999 · 1.1K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

VSV strains with defects in their ability to shutdown innate immunity are potent systemic anti-cancer agents

David F. Stojdl, Brian D. Lichty, Benjamin R. tenOever et al. · Cancer Cell · 2003 · 848 citations · Full text

Life Sciences, Immunology, Oncology +14

Exploiting tumor-specific defects in the interferon pathway with a previously unknown oncolytic virus

David F. Stojdl, Brian D. Lichty, Shane Knowles et al. · Nature Medicine · 2000 · 816 citations

Life Sciences, Molecular Biology, Oncology +16