Concepedia

A new procedure for purifying histone pairs H2A+H2B and H3+H4 from chromatin using hydroxylapatite

Richard H. Simon, Gary Felsenfeld

Nucleic Acids Research · 1979 · 326 citations · 14 references

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Concepts

Abstract

A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA.

References

14

Selective dissociation of histones from calf thymus nucleoprotein

Heiko H. Ohlenbusch, Baldomero M. Olivera, Dorothy Tuan et al. · Journal of Molecular Biology · 1967 · 391 citations

, , +8

The organization of histones and DNA in chromatin: Evidence for an arginine-rich histone kernel

R. Daniel Camerini‐Otero, Barbara Sollner-Webb, Gary Felsenfeld · Cell · 1976 · 370 citations

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Synthesis of Globin Ribonucleic Acid from Duck-Reticulocyte Chromatin <i>In Vitro</i>

Richard Axel, Howard Cedar, Gary Felsenfeld · Proceedings of the National Academy of Sciences · 1973 · 214 citations · Full text

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Chromatin structure as probed by nucleases and proteases: Evidence for the central role of hitones H3 and H4

Barbara Sollner-Webb, R. Daniel Camerini‐Otero, Gary Felsenfeld · Cell · 1976 · 200 citations

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A new procedure for the isolation and fractionation of histones

D R van der Westhuyzen, Claus von Holt · FEBS Letters · 1971 · 152 citations

, , +12