Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases

A serine protease triad forms the catalytic centre of a triacylglycerol lipase

Leo Brady, A.M. Brzozowski, Zygmunt S. Derewenda et al. · Nature · 1990 · 1.2K citations

Life Sciences, Biochemistry, Molecular Biology +16

Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes

JoséM. Guisán · Enzyme and Microbial Technology · 1988 · 600 citations

Life Sciences, Biochemistry, Biotechnology +14

A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports

Ágatha Bastida, Pilar Sabuquillo, Pilar Armisén et al. · Biotechnology and Bioengineering · 1998 · 511 citations

Life Sciences, Biochemistry, Biotechnology +17

The coimmobilization of d-amino acid oxidase and catalase enables the quantitative transformation of d-amino acids (d-phenylalanine) into α-keto acids (phenylpyruvic acid)

Roberto Fernández‐Lafuente, V. Rodríguez, José M. Guisán · Enzyme and Microbial Technology · 1998 · 141 citations

Life Sciences, Biochemistry, Biotechnology +11

Immobilization‐stabilization of α‐chymotrypsin by covalent attachment to aldehyde‐agarose gels

José M. Guisán, Ágatha Bastida, Carmen Cuesta et al. · Biotechnology and Bioengineering · 1991 · 136 citations

Life Sciences, Biochemistry, Biotechnology +17