Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation

TLDR

The paper presents practical guidelines for preparing tissue sections for direct MALDI mass spectrometry analysis. The authors describe sample handling steps, matrix optimization, application methods, instrument settings, and advanced data analysis needed for direct tissue MALDI MS. © 2003 John Wiley & Sons, Ltd.

Abstract

Abstract Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.