Analytical Chemistry · 2004 · 284 citations · 32 references
PharmacyBiochemistryPharmacologyAnalytical ChemistryMass Spectrometric AnalysisMolecular ImagingIonization TechniquesLipidomicsMass AnalyzersMass Spectrometric ImagingMass SpectrometryPeptide AnalysisMolecular Ion SpectroscopySecondary Ion Mass SpectrometrySecondary Ion Mass Spectrometry ImagingSecondary Ion ProductionTime-of-flight Mass AnalyzersTime-of-flight Mass SpectrometryTime-of-flight Secondary Ion Mass SpectrometryTissue Imaging
The use of gold cluster focused ion beams produced by a liquid metal ion gun in a TOF-SIMS mass spectrometer is shown to dramatically enhance secondary ion emission of phospholipids and peptides. The method has been successfully tested with cells grown onto plastic slips and with mouse brain slices, without any treatment of the samples. Very reliable time-of-flight mass spectra are acquired with a low primary ion dose of a few 10(7) ions, and high lateral resolution molecular ion images are obtained for heavy ions of great biological interest. This approach offers new opportunities in pharmacological and biological research fields by localizing compounds of interest such as drugs or metabolites in tissues.
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Daniel E. Weibel, Steve Wong, Nicholas P. Lockyer et al. · Analytical Chemistry · 2003 · 539 citations
Cholesterol as Modulator of Receptor Function
Gerald Gimpl, Katja Bürger, Falk Fahrenholz · Biochemistry · 1997 · 456 citations