A high‐molecular‐mass cell wall protein released from <i>Clostridium tyrobutyricum</i> by heat treatment

Abstract

Analysis of the cell wall of 4 strains of Clostridium tyrobutyricum reveals an unusually high protein content (35-40% dry weight). Brief heat treatment of whole cells of these stains causes release of two proteins, flagellin and a cell wall component of high molecular mass (110-125 kDa in the different strains). This component represents approx. 5% of the dry cell weight.