A micromethod for the stereospecific determination of triglyceride structure

Abstract

Triglyceride lipase and diglyceride kinase can be used in a sensitive stereospecific analysis of the separate fatty acid compositions at the 1, 2 and 3 positions of a triglyceride.Diglyceride kinase fromEscherichia coli selectively catalyzes the phosphorylation of 1,2-diglycerides but not the 2,3-diglycerides.The composition of the 3-position in rat liver triglycerides is clearly different from that at the 1-position.