Organic Letters · 2013 · 27 citations · 12 references
Natural SciencesBiophysicsChemistryBiophysical ChemistryNucleic Acid ChemistrySpectroscopyDna ChemistryFluorescence MicroscopyFluorescence SpectroscopyNucleic Acid BiophysicsOptical SpectroscopyPhotophysics Of Fluorescent ProbesElectronic Structure AnalysisFluorescence ProbesFluorescent DyesFluorescent ProbesFluorescent StainingLinear DichroismLive-cell Imaging ProbesOptical Emission Spectroscopy
DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.
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