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Rapid determination of acyl chain position in egg phosphatidylcholine by high performance liquid chromatography/electrospray mass spectrometry
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1998
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Glycerol BackboneLipid AnalysisEngineeringFood ChemistryBioanalysisAnalytical ChemistryClinical ChemistryAcyl Chain PositionChromatographyBiophysicsRapid DeterminationBiochemistryOne-step MethodEgg PhosphatidylcholineChromatographic AnalysisBiomolecular EngineeringMass SpectrometryLipid ChemistryMedicineDrug Analysis
A simple, one-step method has been developed for the analysis of the components of egg phosphatidylcholine. The method allows, in one run, (a) determination of the total number of carbon atoms and double bonds in the acyl chains of individual components in the mixture and (b) unequivocal assignment of the position of the acyl chains on the glycerol backbone. The presence of degradation products and impurities can be established in the same run. © 1998 John Wiley & Sons, Ltd.