<i>Escherichia coli</i>Transformant Expressing the Glucose Dehydrogenase Gene from<i>Bacillus megaterium</i>as a Cofactor Regenerator in a Chiral Alcohol Production System

Abstract

Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.