A synthetic biology approach for the transformation of <scp>l</scp>-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Abstract

Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.