Concepedia

Compact, hydrophilic, lanthanide-binding tags for paramagnetic NMR spectroscopy

Michael D. Lee, Choy‐Theng Loh, Jung‐Min Shin, Sandeep Chhabra, Matthew L. Dennis, Gottfried Otting, James Swarbrick, Brendan F. Graham

Chemical Science · 2015 · 40 citations · 54 references

DOIFull text

Open access

Concepts

Abstract

tags' capacity to induce PCSs was further demonstrated on GB1 Q32C and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) S112C/C80A mutants. Whilst factors such as the choice of lanthanide ion, pH and site of conjugation influence the size of the PCSs obtained, the tags represent a significant advance in the field.

References

54

Two Crystal Structures of the B1 Immunoglobulin-Binding Domain of Streptococcal Protein G and Comparison with NMR

Travis Gallagher, Patrick Alexander, Philip N. Bryan et al. · Biochemistry · 1994 · 428 citations

, , +17

NMR: prediction of molecular alignment from structure using the PALES software

Markus Zweckstetter · Nature Protocols · 2008 · 372 citations

, , +14

Conformational and Coordination Equilibria on DOTA Complexes of Lanthanide Metal Ions in Aqueous Solution Studied by <sup>1</sup>H-NMR Spectroscopy

Silvio Aime, Mauro Botta, Mauro Fasano et al. · Inorganic Chemistry · 1997 · 363 citations · Full text

, , +15

Thermodynamic study of lanthanide complexes of 1,4,7-triazacyclononane-N,N',N"-triacetic acid and 1,4,7,10-tetraazacyclododecane-N,N',N",N'''-tetraacetic acid

William P. Cacheris, S. K. Nickle, A. Dean Sherry · Inorganic Chemistry · 1987 · 318 citations

, , +13

Lanthanide-Induced Pseudocontact Shifts for Solution Structure Refinements of Macromolecules in Shells up to 40 Å from the Metal Ion

Marco Allegrozzi, Ivano Bertini, Matthias Janik et al. · Journal of the American Chemical Society · 2000 · 215 citations

, , +17