Expanded bed protein A affinity chromatography of a recombinant humanized monoclonal antibody: process development, operation, and comparison with a packed bed method

Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose

Peter L. Ey, Stephen J. Prowse, C. R. Jenkin · Immunochemistry · 1978 · 2.7K citations

Life Sciences, Biochemistry, Biotechnology +13

Humanization of an anti-p185HER2 antibody for human cancer therapy.

Paul Carter, Leonard G. Presta, C Gorman et al. · Proceedings of the National Academy of Sciences · 1992 · 1.9K citations

Pharmacy, Molecular Biology, Oncology +16

Phase II study of weekly intravenous recombinant humanized anti-p185HER2 monoclonal antibody in patients with HER2/neu-overexpressing metastatic breast cancer.

José Baselga, Debu Tripathy, John Mendelsohn et al. · Journal of Clinical Oncology · 1996 · 1.4K citations

Medicine, Oncology, Pharmacology +17

Single–Step Recovery of a Secreted Recombinant Protein by Expanded Bed Adsorption

Marianne Hansson, Stefan Ståhl, Rolf Hjorth et al. · Bio/Technology · 1994 · 139 citations

Engineering, Bioengineering, Biotechnology +13

Comparison of immunoglobulin binding capacities and ligand leakage using eight different protein A affinity chromatography matrices

Paul Füglistaller · Journal of Immunological Methods · 1989 · 138 citations

Life Sciences, Biochemistry, Immunology +17