Incidence of Viruses and Virus like Diseases of Sweetpotato in Uganda

TLDR

Researchers surveyed 1,260 sweetpotato plants across Uganda’s four agroecological zones, using serology and IC‑RT‑PCR to detect SPCSV, SPFMV, SPMMV, and SPCFV (the latter lacking sequence data). Virus incidence ranged from 2.7 % to 20 % across districts, with SPCSV and SPFMV present in all 14 districts, frequent co‑occurrence of SPCSV/SPFMV/SMMV, and co‑infections associated with more severe symptoms; 11 % of symptomatic plants tested negative, suggesting additional viruses.

Abstract

Sweetpotato plants were surveyed for viruslike diseases and viruses in the four major agroecological zones of Uganda. Testing of 1,260 sweetpotato plants, of which 634 had virus-like symptoms, showed that virus disease incidence ranged from 2.7% (Soroti district, short grassland—savannah zone) to 20% (Mukono district, tall grass—forest mosaic zone). Sweet potato chlorotic stunt virus (SPCSV), Sweet potato feathery mottle virus (SPFMV), Sweet potato mild mottle virus (SPMMV), and sweet potato chlorotic fleck virus (SPCFV) were serologically detected and positive results confirmed by immunocapture reverse transcriptase polymerase chain reaction (IC-RT-PCR) and subsequent sequence analyses of the amplified fragments, except SPCFV, which lacked sequence information. SPCSV and SPFMV were detected in all the 14 districts surveyed, whereas SPMMV and SPCFV were detected in 13 and 8 districts, respectively. Logistic regression analysis revealed that SPCSV and SPFMV, SPFMV and SPMMV, and SPFMV and SPCFV more frequently occurred together than any other virus combinations or as single virus infections. Co-infections of SPCSV with SPFMV and/or SPMMV were associated with more severe and persistent symptoms than infections with each of the viruses alone. Several plants (11%) displaying viruslike symptoms did not react with the virus antisera used, suggesting that more viruses or viruslike agents are infecting sweetpotatoes in Uganda.