Biocontrol traits of two <i>Paenibacillus polymyxa</i> strains SQR‐21 and WR‐2 in response to fusaric acid, a phytotoxin produced by <i>Fusarium</i> species

Abstract

The aim of the present study was to investigate the effects of the phytotoxin fusaric acid ( FA ) on the biocontrol traits of two biocontrol strains Paenibacillus polymyxa WR ‐2 and SQR ‐21. The results showed that the growth of both WR ‐2 and SQR ‐21 decreased with increasing FA concentration, and at 70 and 80 μ g mL −1 FA , respectively, the strains were unable to grow. The biocontrol traits of both strains were negatively affected by FA concentration higher than 2·5 μ g mL −1 . However, at 2·5 μ g mL −1 FA , biofilm formation and root colonization were not affected and there was even a positive effect on the production of spores and hydrolytic enzymes (protease and β ‐l,3‐glucanase). The production of fusaricidin‐type antifungal compounds was increased with an increase in FA concentration up to 50 and 60 μ g mL −1 for WR ‐2 and SQR ‐21, respectively. The production of antifungal volatile organic compounds by WR ‐2 and SQR ‐21 was increased only at 2·5 μ g mL −1 FA . The effect of FA on the overall metabolic activity of WR ‐2 and SQR ‐21 was also determined. This study will help to understand the response of P. polymyxa strains to FA and will help to improve their biocontrol efficiency.