Cloning and expression of a cysteine-rich venom protein from Trimeresurus mucrosquamatus (Taiwan Habu)

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

Andrew P. Feinberg, Bert Vogelstein · Analytical Biochemistry · 1983 · 25.2K citations

Life Sciences, Genetics, Molecular Biology +7

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

John M. Chirgwin, Alan Przybyla, Raymond J. MacDonald et al. · Biochemistry · 1979 · 22.2K citations

Life Sciences, Biochemistry, Molecular Biology +9

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

W. Neal Burnette · Analytical Biochemistry · 1981 · 8.5K citations

Life Sciences, Biochemistry, Molecular Biology +15

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Michael A. Frohman, Michael Dush, Gail R. Martin · Proceedings of the National Academy of Sciences · 1988 · 4.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +13