Bioscience Biotechnology and Biochemistry · 2006 · 38 citations · 8 references
Life SciencesBiochemistryMolecular BiologyEnzymologyPrion DiseasesDegradation ProductsEnzyme KineticsEnzyme StabilityPrion Protein MisfoldingProtein DegradationProteolytic EnzymesOptimal Temperature RangePrion StabilityProtease InactivationProtease-mediated Protein DegradationProteinase K-resistant Prion ProteinProteolysis KineticsProteolytic ActivityProteolytic Stability
A keratinolytic alkaline proteae (NAPase) from Nocardiopsis sp. TOA-1 degraded a scrapie prion without any chemical or physical treatment. Optimal temperature and pH were 60 degrees C and above pH 10.0. The scrapie prion was completely degraded within 3 min under optimal conditions.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations