Synthesis and Evaluation of Chromogenic and Fluorogenic Analogs of Glycerol for Enzyme Assays

Abstract

Abstract The branched glycerol analogs 1 and 2 were prepared. Mono‐ester derivatives of these triols undergo a chromogenic or fluorogenic reaction in the presence of NaIO 4 . In contrast, both the diesters and the triols are themselves not chromogenic or fluorogenic. Diester derivatives of these triols can be used as probes for lipases. The tris‐phosphate derivative of 1 is a fluorogenic substrate for various phosphatases.