Scandinavian Journal of Clinical and Laboratory Investigation · 1953 · 1.1K citations · 4 references
Life SciencesBiochemistryAnalytical ChemistryClinical BiochemistryProtein BiochemistryFluid AnalysisProtein AnalysisProtein CharacterizationProtein QuantificationCerebrospinal Fluid AnalysisCerebrospinal Fluid DiagnosticsProtein Analysis In Body FluidsProtein Content Determination
AbstractA rapid and convenient colorimetric method for the determination of small amounts of protein, based on the biuret reaction and readings at 330 mμ, is described. The "micromethod" allows the determination of 10 to 200 mg per cent protein with an acurassy of ± 1.5 mg per cent. An "ultra micro" modification allows determinations of 10 to 400 micrograms protein.The method is applied for the determination of total protein in 1 ml of cerebrospinal fluid.
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