Quantitative Gstp1 Methylation Clearly Distinguishes Benign Prostatic Tissue And Limited Prostate Adenocarcinoma

Abstract

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Mar 2003Quantitative Gstp1 Methylation Clearly Distinguishes Benign Prostatic Tissue And Limited Prostate Adenocarcinoma SUSAN V. HARDEN, ZHONGMIN GUO, JONATHAN I. EPSTEIN, and DAVID SIDRANSKY SUSAN V. HARDENSUSAN V. HARDEN , ZHONGMIN GUOZHONGMIN GUO , JONATHAN I. EPSTEINJONATHAN I. EPSTEIN , and DAVID SIDRANSKYDAVID SIDRANSKY View All Author Informationhttps://doi.org/10.1097/01.ju.0000049627.90307.4dAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Hypermethylation of the glutathione S-transferase gene (GSTP1) is the most common (greater than 90%) reported epigenetic alteration in prostate cancer. It occurs early in cancer progression and it is a promising marker for detecting organ confined disease. To evaluate its use as a diagnostic tool for cancer we used quantitative GSTP1 methylation to test for the presence of cancer in 45 prostate needle biopsy samples. Materials and Methods: Paraffin tissue samples from 45 patients with minute foci of intermediate grade prostatic adenocarcinoma or benign disease on needle biopsy were tested for GSTP1 hypermethylation using quantitative fluorogenic real-time methylation specific polymerase chain reaction. This assay was performed in blinded fashion without previous knowledge of the histopathological diagnosis. Results: DNA from 29 of the 45 paraffin samples was amenable to polymerase chain reaction amplification. In these 29 samples GSTP1 methylation was detected in 11 of 15 cases of limited cancer and in 0 of 14 of benign disease (2-sided Fisher's exact test, p <0.0001). Thus, this assay had 73% sensitivity, 100% specificity, 100% positive and 78% negative predictive values. 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Google Scholar From the Departments of Otolaryngology-Head and Neck Surgery (Head and Neck Cancer Research Division), Pathology, Urology and Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byStewart G, Van Neste L, Delvenne P, Delrée P, Delga A, McNeill S, O'Donnell M, Clark J, Van Criekinge W, Bigley J and Harrison D (2013) Clinical Utility of an Epigenetic Assay to Detect Occult Prostate Cancer in Histopathologically Negative Biopsies: Results of the MATLOC StudyJournal of Urology, VOL. 189, NO. 3, (1110-1116), Online publication date: 1-Mar-2013.ZHOU M, TOKUMARU Y, SIDRANSKY D and EPSTEIN J (2018) QUANTITATIVE GSTP1 METHYLATION LEVELS CORRELATE WITH GLEASON GRADE AND TUMOR VOLUME IN PROSTATE NEEDLE BIOPSIESJournal of Urology, VOL. 171, NO. 6 Part 1, (2195-2198), Online publication date: 1-Jun-2004. Volume 169Issue 3March 2003Page: 1138-1142 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordstumor markers, biologicalprostatic neoplasmsgene expressionprostateglutathione transferaseMetricsAuthor Information SUSAN V. HARDEN Equal study contribution. More articles by this author ZHONGMIN GUO Equal study contribution. More articles by this author JONATHAN I. EPSTEIN More articles by this author DAVID SIDRANSKY Financial interest and/or other relationship with Virco, Inc. More articles by this author Expand All Advertisement PDF downloadLoading ...