Macromolecular Bioscience · 2011 · 84 citations · 16 references
PharmacyPharmaceutical BiotechnologyPharmaceuticsDrug DeliveryNanoparticle Drug DeliveryPolymer ChemistryDrug Delivery EngineeringLipid-based Drug Delivery SystemsNanoparticle DesignNucleic Acid DeliveryPolymeric Drug Delivery SystemsSmall Interfering Rna DeliveryCellular Uptake ModulationGene Delivery CarriersHydrophobic ModificationIntracellular Delivery Of Nucleic AcidsNanoparticle Cellular UptakeNucleic Acid NanocomplexesPolymer-based Delivery SystemsPolymeric Nanocarriers
Characterization of a polymer library engineered to enhance their ability to protect and deliver their nucleotide cargo to the cells is reported. The ζ-potential continuously increased with higher polymer:siRNA weight ratio, and the ζ-potential of lipid-modified polymers:siRNA complexes were higher than PEI2 at all ratios. At polymer:siRNA ratio of 1:1, all lipid-substituted polymers showed complete protection against degradation. Lipid-modified polymers significantly increased the cellular uptake of siRNA complexes and down-regulation of GAPDH and P-gp (max. 66% and 67%, respectively). The results indicate that hydrophobic modification of low molecular PEI could render this otherwise ineffective polymer to a safe effective delivery system for intracellular siRNA delivery and protein silencing.
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Argonaute2 Is the Catalytic Engine of Mammalian RNAi
Jidong Liu, Michelle A. Carmell, Fabiola V. Rivas et al. · Science · 2004 · 2.7K citations