Cellulolytic Enzyme System of Acetivibrio cellulolyticus, a Newly Isolated Anaerobe

Abstract

Crude enzyme preparations from Acetivibrio cellulolyticus converted ball-milled pulp, cotton batting, filter and tissue paper, a microcrystalline cellulose, carboxymethylcellulose, cellobiose and xylan to reducing sugars. The preparations showed maximum activity between pH 5 and 6 and at a temperature between 37 and 50 °C, depending on the substrate used. The enzyme activity was fairly stable at 2 °C for 4 weeks. The saccharifying ability of the preparation was comparable to that of commercially available cellulase preparations from Aspergillus niger and Trichoderma viride.