Composition, particle size and role in dietary fat transport of two different lipoproteins of the intestinal lymph

Abstract

Abstract Rat intestinal lymph very low density lipoproteins have been separated by cellulose acetate electrophoresis. After administration of a meal including natural fats and labeled fatty acids, two labeled bands were detected on the electrophoregraphs. Further separations of these two kinds of particles were performed by density gradient zonal centrifugation. The isolated lipoproteins were analyzed for chemical composition and investigated by electron microscopy and electrophoresis. The moving particles had a higher protein and cholesterol content and a smaller diameter than the particles which remained at the origin. It has also been shown that the major part of the labeled lymph lipids were transported by the smaller particles.