The high performance liquid chromatographic determination of total malondialdehyde in vegetable oil with dansyl hydrazine

Abstract

Abstract A simple high‐performance liquid chromatographic (h.p.l.c.) method has been developed for determining total malondialdehyde (MDA), including free MDA and MDA released from its precursor in vegetable oils. Free MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1‐dansyl‐pyrazole(DP), was determined by h.p.l.c. The reaction releasing MDA from its precursor requires Fe 3+ in 5% hydrochloric acid medium. Recoveries of free MDA and tetramethoxypropane (used as an MDA precursor) in vegetable oils were satisfactory. During autoxidation, a remarkable difference between the DP values of methyl oleate, methyl linoleate and some vegetable oils, and that of methyl linolenate was observed. Only methyl linolenate released MDA during autoxidation. For autoxidised methyl linolenate the DP value correlated with the thiobarbituric acid(TBA) value, although the DP value was only 30% of the TBA value.