DNA-labeled clay: A sensitive new method for tracing particle transport

Abstract

Research Article| September 01, 1998 DNA-labeled clay: A sensitive new method for tracing particle transport Barbara J. Mahler; Barbara J. Mahler 1Department of Geological Sciences, University of Texas, Austin, Texas 78712 Search for other works by this author on: GSW Google Scholar Matthew Winkler; Matthew Winkler 2Ambion Incorporated, 2130 Woodward Street, Austin, Texas 78744 Search for other works by this author on: GSW Google Scholar Philip Bennett; Philip Bennett 1Department of Geological Sciences, University of Texas, Austin, Texas 78712 Search for other works by this author on: GSW Google Scholar David M. Hillis David M. Hillis 3Department of Zoology and Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712 Search for other works by this author on: GSW Google Scholar Geology (1998) 26 (9): 831–834. https://doi.org/10.1130/0091-7613(1998)026<0831:DLCASN>2.3.CO;2 Article history first online: 02 Jun 2017 Cite View This Citation Add to Citation Manager Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Search Site Citation Barbara J. Mahler, Matthew Winkler, Philip Bennett, David M. Hillis; DNA-labeled clay: A sensitive new method for tracing particle transport. Geology 1998;; 26 (9): 831–834. doi: https://doi.org/10.1130/0091-7613(1998)026<0831:DLCASN>2.3.CO;2 Download citation file: Ris (Zotero) Refmanager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentBy SocietyGeology Search Advanced Search Abstract The behavior of mobile colloids and sediment in most natural environments remains poorly understood, in part because characteristics of existing sediment tracers limit their widespread use. Here we describe the development of a new approach that uses a DNA-labeled montmorillonite clay as a highly sensitive and selective sediment tracer that can potentially characterize sediment and colloid transport in a wide variety of environments, including marine, wetland, ground-water, and atmospheric systems. Characteristics of DNA in natural systems render it unsuitable as an aqueous tracer but admirably suited as a label for tracing particulates. The DNA-labeled-clay approach, using techniques developed from molecular biology, has extremely low detection limits, very specific detection, and a virtually infinite number of tracer signatures. Furthermore, DNA-labeled clay has the same physical characteristics as the particles it is designed to trace, it is environmentally benign, and it can be relatively inexpensively produced and detected. Our initial results show that short (500 base pair) strands of synthetically produced DNA reversibly adsorb to both Na-montmorillonite and powdered silica surfaces via a magnesium bridge. The DNA-montmorillonite surface complexes are stable in calcium-bicarbonate spring waters for periods of up to 18 days and only slowly desorb to the aqueous phase, whereas the silica surface complex is stable only in distilled water. Both materials readily release the adsorbed DNA in dilute EDTA solutions for amplification by the polymerase chain reaction (PCR) and quantification. The stability of the DNA-labeled clay complex suggests that this material would be appropriate for use as an extremely sensitive sediment tracer for flow periods of as long as 2 weeks, and possibly longer. This content is PDF only. Please click on the PDF icon to access. First Page Preview Close Modal You do not have access to this content, please speak to your institutional administrator if you feel you should have access.