Secretion of functional anti-CD30-angiogenin immunotoxins into the supernatant of transfected 293T-cells

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

Life Sciences, Molecular Biology, Molecular Genetics +11

Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5

Frank L. Graham, W. C. Russell, James R. Smiley et al. · Journal of General Virology · 1977 · 4.9K citations

Life Sciences, Molecular Biology, Molecular Genetics +16

Metal chelate affinity chromatography, a new approach to protein fractionation

Jerker Porath, Jan Carlsson, Ingmar Olsson et al. · Nature · 1975 · 2.1K citations

Life Sciences, Biochemistry, Chemistry +14

A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies

Johannes Büchner, Ira Pastan, Ulrich Brinkmann · Analytical Biochemistry · 1992 · 342 citations

Life Sciences, Biotechnology, Molecular Biology +12

Protein Misfolding and Inclusion Body Formation in Recombinant Escherichia coli Cells Overexpressing Heat-shock Proteins

Jeffrey G. Thomas, François Baneyx · Journal of Biological Chemistry · 1996 · 224 citations · Full text

Life Sciences, Microbiology, Molecular Biology +17