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Genotyping of Toxoplasma gondii strains detected in pork sausage

Aristeu Vieira da Silva, André de Oliveira Mendonça, Sandia Bergamaschi Pezerico, Paulo Francisco Domingues, Hélio Langoni

Parasitología latinoamericana · 2005 · 26 citations · 16 references

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Abstract

DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments in the city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples. Digestion of amplicons with restriction enzymes HhaI and Sau3AI showed that 14 (73.68%) samples contained parasites genotyped as Type I, and 5 (26.32%) as Type III.

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