FOR20, a conserved centrosomal protein, is required for assembly of the transition zone and basal body docking at the cell surface

Abstract

Within the FOP family of centrosomal proteins, the conserved FOR20 protein has been implicated in the control of primary cilium assembly in human cells. To ascertain its role in ciliogenesis, we have investigated the function of its ortholog, PtFOR20p, in a multiciliated unicellular organism, Paramecium. By a combined functional and cytological analysis, we found that PtFOR20p specifically localizes at basal bodies and is required to build the transition zone, a prerequisite to their maturation and docking at the cell surface, hence to ciliogenesis. We also found that PtCen2p (one of the two basal body specific centrins, ortholog of HsCen2) is required to recruit PtFOR20p at the developing basal body and to control its length. In contrast, the other basal body specific centrin, PtCen3p, is not needed for assembly of the transition zone, but is required downstream, for basal body docking. Comparison of the structural defects induced by depletion of PtFOR20p, PtCen2p or PtCen3p respectively illustrates the dual role of the transition zone in the biogenesis of the basal body and in cilium assembly. The multiple potential roles of the transition zone during basal body biogenesis and the evolutionary conserved function of the FOP proteins in microtubule membrane interactions are discussed.