The Use of ¹³C–NMR. Spectroscopy in Biosynthetic Studies. II[1]. Biosynthesis of narasin, a new polyether ionophore from fermentation of <i>Streptomyces aureofaciens</i> [2]

Abstract

Abstract The labeling of the polyether ionophore, narasin, using 13 C‐labeled acetate, propionates, and butyrates in the course of fermentation of Streptomyces aureofacies resulted in the isolation of differently labeled compounds. A careful examination of the 13 C‐NMR. spectral data indicated derivation of narasin from five acetate, seven propionate, and three butyrate units. The evidence of indirect incorporation of 13 C led to the first documented demonstration of conversion of propionate to butyrate in the course of the synthesis of narasin by S. aureofaciens .