A valid ELISPOT assay for enumeration of ex vivo, antigen-specific, IFNγ-producing T cells

Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.

Holly Cherwinski, J H Schumacher, Kenneth D. Brown et al. · The Journal of Experimental Medicine · 1987 · 1K citations · Full text

Life Sciences, Immunology, Molecular Biology +14

Reverse ELISPOT assay for clonal analysis of cytokine production I. Enumeration of gamma-interferon-secreting cells

Cécil Czerkinsky, Gudrun Andersson, Hans-Peter T. Ekre et al. · Journal of Immunological Methods · 1988 · 496 citations

Life Sciences, Immunology, Molecular Biology +12

Quantification of antigen specific CD8+ T cells using an ELISPOT assay

Yasushi Miyahira, Kenichiro Murata, Dolores Rodrı́guez et al. · Journal of Immunological Methods · 1995 · 364 citations · Full text

Life Sciences, Cell Biology, Immunology +17

Mycobacterial Dose Defines the Th1/Th2 Nature of the Immune Response Independently of Whether Immunization Is Administered by the Intravenous, Subcutaneous, or Intradermal Route

Carl A. Power, Guojian Wei, Peter A. Bretscher · Infection and Immunity · 1998 · 186 citations · Full text

Life Sciences, Immunology, Microbiology +16

Detection of individual mouse splenic T cells producing IFN-γ and IL-5 using the enzyme-linked immunospot (ELISPOT) assay

Takashi Taguchi, Jerry R. McGhee, Robert L. Coffman et al. · Journal of Immunological Methods · 1990 · 166 citations

Life Sciences, Immunology, Cellular Immunology +10