European Journal of Immunology · 2005 · 87 citations · 33 references
Life SciencesImmunologyNeuroscienceAutoimmunityInflammationNeuroimmunologyCentral Nervous System ImmunityImmune SignalingIon Channel PharmacologyIon Channel RegulationNeuroimmune RegulationNeuroinflammationChannelsExperimental Autoimmune EncephalomyelitisIon Channel SelectivityPotassium Channel BlockersPotassium Channel PhysiologyPro-inflammatory Mediator ProductionT Cell Effector FunctionsT Helper Cell Effector Functions
The KCNN4 potassium-ion channel has been reported to play an important role in regulating antigen-induced T cell effector functions in vitro. This study presents the first evidence that a selective KCNN4 blocker, TRAM-34, confers protection against experimental autoimmune encephalomyelitis (EAE) in the mouse model. Treatment with the KCNN4 blocker did not prevent infiltration of T cells in the spinal cord, but resulted in the reduction of both the protein and the message levels of TNF-alpha and IFN-gamma as well as the message levels of several other pro-inflammatory molecules in the spinal cord. Plasma concentrations of TRAM-34 within a 24-h period were between the in vitro IC(50) and IC(90) values for the KCNN4 channel. The effect of TRAM-34 was reversible, as indicated by the development of clinical EAE symptoms within 48 h after withdrawal of treatment. In summary, our data support the idea that KCNN4 channels play a critical role in the immune response during the development of MOG-induced EAE in C57BL/6 mice.
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Heike Wulff, Mark J. Miller, Wolfram Hänsel et al. · Proceedings of the National Academy of Sciences · 2000 · 606 citations · Full text
Itzhack Mendel, Nicole Kerlero de Rosbo, Avraham Ben‐Nun · European Journal of Immunology · 1995 · 604 citations