REDUCTIVE ALKYLATION OF PROTEINS WITH AROMATIC ALDEHYDES AND SODIUM CYANOBOROHYDRIDE

Abstract

Reductive alkylation of amino groups in proteins is achieved by treatment with substituted nitrobenzaldehydes and sodium cyanoborohydride in an alcoholic pH 5.2 lithium acetate buffer. The extent of modification was observed by UV spectroscopy and amino acid analysis. Because the new reductive alkylation can be carried out under mild conditions, it should be useful in protein chemistry.