Changes in membrane lipid components and antioxidant levels during natural ageing of seeds of <i>Acer platanoides</i>

Abstract

Changes in membrane lipid components and cellular antioxidant systems were investigated through 7 years in seeds of Acer platanoides L. after storage in natural conditions, i.e. ‐ 3°C and 10% water content. The loss of germination capacity in aged seeds was associated with increased solute leakage during imbibition, reduced content of phospholipids, especially phosphatidylcholine, and increased free fatty acid content. A marked decrease of unsaturated fatty acids in the phospholipid fraction was observed after one year of storage. Antioxidant potential in the lipid fraction and level of ‐SH groups decreased during storage. The results are consistent with the hypothesis that ageing in seeds is mediated by a free radical mechanism.