ISOELECTROPHORETIC SEPARATION OF COMPLEX NITROGENOUS SUBSTANCES OF BEER AND BREWING MATERIALS

Abstract

Complex nitrogenous materials extracted from barley, malt, wort and beer have been separated by isoelectrophoresis in thin layers of Sephadex gel. Comparison and interpretation of the different isoelectric profiles has been attempted. A preparative isoelectrophoretic procedure has been developed. The isoelectric species of beer have been isolated in quantities sufficient for certain of their physical and chemical properties to be determined. It is concluded that substances with typical protein-like properties account for only about one-third (by weight) of the total complex-nitrogen fraction of beer. An arbitrary but useful classification of the complex-nitrogen fraction of beer is suggested.