Selective removal of transcription-blocking DNA damage from the transcribed strand of the mammalian DHFR gene

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Peter Rigby, Marianne Dieckmann, Carl Rhodes et al. · Journal of Molecular Biology · 1977 · 11.7K citations

Life Sciences, Molecular Biology, Molecular Genetics +14

Efficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +11

Chromosomal Subunits in Active Genes Have an Altered Conformation

Harold Weintraub, Mark Groudine · Science · 1976 · 1.8K citations

Life Sciences, Biochemistry, Genetics +9