Experimental and Clinical Endocrinology & Diabetes · 2009 · 19 citations · 0 references
Life SciencesBiochemistryPharmacologyNeurochemistryNeuropharmacologyProtein BiochemistryAmino Acid MetabolismPeptide AnalysisPeptide ChemistryProtein DegradationProteolytic EnzymesProteolytic ProcessingAmino AcidsHigh-performance Liquid ChromatographyNeuropeptide DegradationNeuropeptide ProcessingPeptide DegradationPeptide Degradation AnalysisProteolytic Degradation Of PeptidesTyrosine Metabolism
The degradation of opioid peptides by human placental aminopeptidase M was studied by measuring liberated amino acids by high performance liquid chromatography. The purified placental aminopeptidase M actively degraded Met-enkephalin (Met-Enk) and Leu-enkephalin (Leu-Enk) completely into constituent 5 amino acids. The activities as a function of tyrosine liberation were observed for Met-Enk followed by Leu-Enk, beta-neoendorphin, dynorphin, and beta-endorphin.