Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

Plasmid‐encoded protein: The principal factor in the “metabolic burden” associated with recombinant bacteria

William E. Bentley, N. Mirjalili, Dana C. Andersen et al. · Biotechnology and Bioengineering · 1990 · 538 citations

Life Sciences, Genetics, Microbiology +16

Determination of plasmid copy number by fluorescence densitometry

Steven J. Projan, Stephen Carleton, Richard P. Novick · Plasmid · 1983 · 287 citations

Life Sciences, Genetics, Molecular Biology +12

Microbial energetics should be considered in manipulating metabolism for biotechnological purposes

A. H. Stouthamer, Henk W. van Verseveld · Trends in biotechnology · 1987 · 59 citations

Life Sciences, Biotechnology, Microbiology +14