Large-scale bacterial fermentation and isolation of scFv multimers using a heat-inducible bacterial expression vector

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

High volumetric yields of functional dimeric miniantibodies in Escherichia coli , using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions

Uwe Horn, Wolfgang Strittmatter, Anke Krebber et al. · Applied Microbiology and Biotechnology · 1996 · 157 citations

Life Sciences, Biotechnology, Microbiology +17

Single-chain Fv fragments of anti-neuraminidase antibody NC10 containing five- and ten-residue linkers form dimers and with zero- residue linker a trimer

Alexander A. Kortt, Myoung Soo Lah, Geoffrey W. Oddie et al. · Protein Engineering Design and Selection · 1997 · 132 citations · Full text

Pharmacy, Immunology, Molecular Biology +15

Recombinant anti‐sialidase single‐chain variable fragment antibody

Alexander A. Kortt, Robyn Malby, J. Bruce Caldwell et al. · European Journal of Biochemistry · 1994 · 128 citations

Life Sciences, Biochemistry, Molecular Biology +17