Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

Christopher K. Savile, Jacob M. Janey, Emily C. Mundorff, +10

Science

EngineeringBiocatalytic Asymmetric SynthesisOrganic ChemistryPharmaceutical SynthesisMedicinal Chemistry +15

Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

Anke Krebber, Susanne Bornhauser, Jörg Burmester, +4

Journal of Immunological Methods

Reliable CloningMedicineSpleen CellImmunologyImmunodominance +8

Competition BIAcore for Measuring True Affinities: Large Differences from Values Determined from Binding Kinetics

Lars Nieba, Anke Krebber, Andreas Plückthun

Analytical Biochemistry

Biophysical ModelingEngineeringBiochemistryMeasuring True AffinitiesMedicine +7

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

Jon E. Ness, Seran Kim, Andrea Gottman, +6

Nature Biotechnology

Functional Protein DiversityMolecular DiversityEngineeringAmino AcidsProtein Folding +9

High volumetric yields of functional dimeric miniantibodies in Escherichia coli , using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions

Uwe Horn, Wolfgang Strittmatter, Anke Krebber, +7

Applied Microbiology and Biotechnology

Functional Dimeric MiniantibodiesEngineeringBiochemistryNatural SciencesBacteriology +9

Highly Enantioselective Reduction of a Small Heterocyclic Ketone: Biocatalytic Reduction of Tetrahydrothiophene-3-one to the Corresponding (<i>R</i>)-Alcohol

Jack Liang, Emily C. Mundorff, Rama Voladri, +8

Organic Process Research & Development

EngineeringChiral PoolOrganic ChemistryChemistryEnzymatic Modification +16

Characterization of Novel Antimicrobial Peptoids

Bob Goodson, Anton F. Ehrhardt, Simon Ng, +10

Antimicrobial Agents and Chemotherapy

Membrane DisruptionAntibioticsPeptoid Chir29498MedicinePeptoid +9

Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes

SunAi Raillard, Anke Krebber, Yonghong Chen, +10

Chemistry & Biology

Homologous EnzymesCellular EnzymologyBiochemistryProtein FoldingNatural Sciences +6

Specific Detection of His-Tagged Proteins with Recombinant Anti-His Tag scFv-Phosphatase or scFv-Phage Fusions

Peter Lindner, Konrad Bauer, Anke Krebber, +7

BioTechniques

Immunocytochemical TechniqueImmunologyMolecular BiologyViral Structural ProteinImmunotherapy +19

Selectively-infective phage (SIP): a mechanistic dissection of a novel in vivo selection for protein-ligand interactions

Claus Krebber, Stefania Spada, Dominique Desplancq, +3

Journal of Molecular Biology

Mechanistic DissectionPeptide LibraryBacteriophageMolecular BiologySelectively-infective Phage +3