Recovery of an Extracellular Alkaline Protease From Whole Fermentation Broth Using Reversed Micelles

Abstract

Abstract The liquid‐liquid extraction of an extracellular alkaline protease from its fermentation broth was carried out, using a reversed micellar organic solvent. Optimum process parameters were determined for extraction using a 250 mM AOT in isooctane micellar solution. With unequal phase volume ratios, purification factors as high as 6 have been obtained from completely untreated broth. Yields up to 56% were achieved from the same broth using a three‐stage cascade.