JAIDS Journal of Acquired Immune Deficiency Syndromes · 2005 · 78 citations · 16 references
Life SciencesImmunologyInfectious DiseasesCellular ImmunologyImmunology Of Infectious DiseasesImmunoregulationAntiretroviral TherapyCellular Immunity In InfectionImmunodeficiency DisordersRegulatory T Cell DysfunctionRegulatory T CellsT Cell BiologyFlow Cytometry ImmunophenotypingFoxp3 BiologyFoxp3 DeficiencyHighly Active Antiretroviral TherapyHiv Antiretroviral TherapyHiv-associated ImmunosuppressionImmune Reconstitution After Antiretroviral TherapyT-cell Immune Responses
The impact of HIV infection on regulatory CD4(+)CD25(high) (Treg) lymphocyte subpopulations was evaluated by FOXP3 quantitative reverse transcriptase polymerase chain reaction and by flow cytometry. FOXP3 mRNA was quantified in peripheral blood mononuclear cells or purified CD4(+) lymphocytes from HIV(+) lymphopenic patients. Patients were distributed among clinical stages A, B, and C and received highly active antiretroviral therapy. The frequency of CD4(+)CD25(high) lymphocytes, measured by flow cytometry, was decreased in HIV patients (n = 38) compared with the group of uninfected subjects (n = 39). FOXP3 mRNA levels were found decreased in HIV patients (n = 25) compared with controls (n = 17) when expression of CD3gamma or beta-actin but not that of TATA box binding protein 1 was used for data normalization. Our results are compatible with a decrease of the Treg lymphocytes during HIV infection. The consequences of a Treg decrease are discussed in the context of immunologic anomalies observed during HIV infection.
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Regulatory T Cell Lineage Specification by the Forkhead Transcription Factor Foxp3
Jason D. Fontenot, Jeffrey P. Rasmussen, Luke M. Williams et al. · Immunity · 2005 · 2.2K citations · Full text
Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection
Tae‐Wook Chun, Lucy M. Carruth, Diana Finzi et al. · Nature · 1997 · 2.1K citations