FEMS Microbiology Letters · 2013 · 31 citations · 32 references
Novel β-GlucosidasesPnpβg Substrateβ-Glucosidase ActivityEngineeringBiochemistryMedicineMicrobial ProteomicsGlycobiologyBiotechnologyEnvironmental MicrobiologyMicrobiologyFood PreservativesCarbohydrate-protein InteractionGlycosylation
An Amazon soil microbial community metagenomic fosmid library was functionally screened for β-glucosidase activity. Contig analysis of positive clones revealed the presence of two ORFs encoding novel β-glucosidases, AmBGL17 and AmBGL18, from the GH3 and GH1 families, respectively. Both AmBGL17 and AmBGL18 were functionally identified as β-glucosidases. The enzymatic activity of AmBGL17 was further characterized. AmBGL17 was tested with different substrates and showed highest activity on pNPβG substrate with an optimum temperature of 45 °C and an optimum pH of 6. AmBGL17 showed a Vmax of 116 mM s(-1) and Km of 0.30 ± 0.017 mM. This is the first report of β-glucosidases from an Amazon soil microbial community using a metagenomic approach.
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Koichiro Tamura, Daniel G. Peterson, Nora Peterson et al. · Molecular Biology and Evolution · 2011 · 40.1K citations · Full text
Maximum Parsimony Methods, Comparative Genomics, Genetics +20
dbCAN: a web resource for automated carbohydrate-active enzyme annotation
Yanbin Yin, Xizeng Mao, Jincai Yang et al. · Nucleic Acids Research · 2012 · 1.9K citations · Full text