Conversion of Red Fluorescent Protein into a Bright Blue Probe

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Natural Sciences, Genetics, Directed Evolution +6

Engineering and characterization of a superfolder green fluorescent protein

J.D. Pédelacq, Stéphanie Cabantous, Timothy H. Tran et al. · Nature Biotechnology · 2005 · 2.4K citations

Protein Assembly, Protein Folding, Natural Sciences +5

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

Andreas Crameri, Erik A. Whitehorn, Emily Tate et al. · Nature Biotechnology · 1996 · 1.2K citations

Dna Sequencing, Molecular Biological Method, Natural Sciences +10

Improving the photostability of bright monomeric orange and red fluorescent proteins

Nathan C. Shaner, Michael Z. Lin, Michael R. McKeown et al. · Nature Methods · 2008 · 1K citations · Full text

Bright Monomeric Orange, Red Fluorescent Proteins, Engineering +10

Quantitative Fluorescence Resonance Energy Transfer Measurements Using Fluorescence Microscopy

Gerald W. Gordon, Gail Berry, Xiao Liang et al. · Biophysical Journal · 1998 · 850 citations · Full text