A versatile coiled-coil tethering system for the oriented display of ligands on nanocarriers for targeted gene delivery

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Otmane Boussif, Frank Lezoualc’h, Maria Antonietta Zanta et al. · Proceedings of the National Academy of Sciences · 1995 · 6.2K citations

Pharmacy, Biotechnology, Pharmaceutics +17

Protein thiolation and reversible protein-protein conjugation. <i>N</i>-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent

J. Carlsson, Håkan Drevin, Rolf Axén · Biochemical Journal · 1978 · 1.2K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

Yves Durocher · Nucleic Acids Research · 2002 · 1.1K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431.

Harry T. Haigler, J. F. Ash, Sherwin J. Singer et al. · Proceedings of the National Academy of Sciences · 1978 · 576 citations · Full text

Life Sciences, Cell Biology, Molecular Biology +15

The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells

Manfred Ogris, Peter Steinlein, Malgorzata Kursa et al. · Gene Therapy · 1998 · 572 citations

Life Sciences, Cell Biology, Molecular Biology +7