A Bioorthogonal Chemoenzymatic Strategy for Defined Protein Dendrimer Assembly

Abstract

Protein dendrimers made easy: We present a chemoenzymatic strategy for the synthesis of well-defined protein dendrimers in which proteins orthogonally labelled by sortase-mediated ligation are conjugated to a dendritic scaffold by azide–alkyne cycloaddition. This “sortase-click” strategy uses readily accessible His6-tagged proteins and can serve as a powerful method for the covalent assembly of multivalent proteins.