Use of Degradation Tags To Control Protein Levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Abstract

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.