Identification of a second locus encoding β-amylase on chromosome 2 of barley

Abstract

Summary A barley endosperm cDNA clone was used to study the polymorphism and chromosomal location of β-amylase genes in barley. Analysis of DNA from seven cultivars digested with three restriction endonucleases showed two types of pattern, one present in Sultan and the other in the remaining six cultivars. A copy-number reconstruction indicated the presence of about three gene copies per haploid genome. Analysis of the six available whole chromosome addition lines and selected telocentric chromosome additions of barley into wheat showed the location of genes on the short arm of chromosome 2 (probably one copy) and the long arm of chromosome 4 (probably two copies).