Engineering Escherichia coli for enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in larger cellular space

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15K citations · Full text

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Natural Sciences, Genetics, Directed Evolution +6

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

Alan H. Rosenberg, Barbara N. Lade, Chui Dao-shan et al. · Gene · 1987 · 1.5K citations

Rna Processing, Natural Sciences, Genetics +11

A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds

Patricia Spiekermann, Bernd H. A. Rehm, Rainer Kalscheuer et al. · Archives of Microbiology · 1999 · 734 citations

Biochemistry, Polyhydroxyalkanoic Acids, Direct Screening +5

Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring

Erfei Bi, Joe Lutkenhaus · Journal of Bacteriology · 1993 · 417 citations · Full text

Ring Structure, Cell Death, Molecular Biology +19