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Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC–MS/MS methods not separating both metabolites as determined by an LC–MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum

MedicineBioanalysisClinical Nutrition25-Hydroxyvitamin D2Analytical Chemistry25-Hydroxyvitamin D3MetabolomicsHuman SerumPharmacologyChromatography